FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036298
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14250.13750870888489605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG6250.025.4997025
GTGTAGT6200.019.8718511
TAGGGCG1850.005071685619.5780669
GTATTAG7850.019.3878971
TATAGGG6151.8189894E-1218.8476962
GTATAGG6301.8189894E-1218.4060461
GACTGGG3201.9412859E-518.1105847
GTATATA25350.017.725351
CAGACCG2050.00917108517.668019
TACACAG6202.910383E-1117.5263715
CTAGACA5001.1155862E-817.3861624
GTGTAGG3804.3978907E-617.164851
GTATATG14400.016.6085831
TTACACA10550.016.4797744
AGACAGG4409.749474E-716.4641696
GTGTATA9250.016.4535141
TCTAGAC4058.094592E-616.0982973
TATACCG5453.229252E-815.9506065
CTTACAC5503.6132406E-815.8056013
GTGTTAA8300.015.7172131
Produced by FastQC (version 0.11.2)