FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848755
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26090.14112199831778682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3851.6370905E-1124.465914145
CTGTGCG2857.1467493E-620.3365369
TAGGTAG7650.019.8879385
GTGTAAG9100.018.3152281
GTATTAG14300.016.7225971
CCTTAAG7703.6379788E-1215.9986751
GTGTAGG6459.476935E-1015.7287471
CTATACA13250.015.3099584
CCAGAGC6651.4642865E-915.2524033
GTATATA34500.015.1230461
TCTATAC12050.015.0308943
GTTATAC16150.014.80380253
TATACCG8351.0913936E-1114.7500785
TAGGACC6401.45137165E-814.7161864
CTATACT13350.014.6525894
CTAGGAC4451.9749115E-514.6525893
CTTACAC8951.8189894E-1214.570733
GCTGTGC5506.218579E-714.4897818
TGTACTG14100.014.3870185
CTGCCGC24800.014.3160515145
Produced by FastQC (version 0.11.2)