FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387994
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49500.11280781149655174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC7750.019.635027145
GTATAGA35100.016.5179521
TACACCG13550.016.0424655
GTATATA72450.015.9049411
TATACAG40900.015.7672465
TAGGACC15200.015.25441554
GACTAGG9500.015.2542428
TATATAC76650.015.0305243
CTGCCGC62600.014.700879145
GTATTAG31300.014.35557651
CCTAATA34850.014.1387212
CCCTAGA16550.014.0128111
TAGGTAG14250.013.7289745
TAGGCCG6051.819124E-613.1742685
ACTATGC30550.013.0447488
GCTGCCC15000.013.043268145
ACCGTGC8402.8012437E-912.9388668
TCTCGGG13550.012.834704145
TACAGGC19450.012.6661187
TAATACT60100.012.6591354
Produced by FastQC (version 0.11.2)