FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132139
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45160.10928964393501768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA28450.019.8650742
CTATGCA20250.019.6790899
ACTATGC34800.018.5301158
TAGGTAG16200.017.8902995
TACTATG30200.017.5141287
ACCTAAT30650.017.2597031
GTATTAG26150.017.1814941
TAATACT56050.016.9345464
GTATAGA27800.016.6830751
CTAATAC32700.016.3969023
TATACAG32200.016.2012755
TATTAGG29150.015.9081342
CTAAGCA21050.015.8337414
GTGTAAG16950.015.8187781
GTATAAA49150.015.7761561
TATACGG18250.015.48368555
GCCGACC8900.015.469194145
AGACTAG13700.015.337347
GTATATA65900.015.1752051
TCTAAGC20800.014.9790053
Produced by FastQC (version 0.11.2)