FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4152471
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47480.11434155711141632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA27850.017.4304712
GTATTAG26800.016.493871
TACTATG28650.016.4377757
TCTAGAC18100.016.4121253
CTAATAC33800.016.2913253
ACTATGC32150.016.2255948
ATACTAT39600.016.100596
TATACAG32100.016.0253525
GTATAGG26700.016.0128361
TAATACT52150.015.8381644
GTATATA67450.015.6855021
TCTCGGG15250.015.6797905145
CGGTGGG21000.015.182018145
TAGGACA18700.014.3355524
TAGACAG24300.014.3116355
TAATAGT47800.014.2480114
GTGTAAG17400.014.15980151
TAGCCCG5155.011678E-614.0684765
TATATAC72450.013.9006763
GTATTAC26700.013.8416041
Produced by FastQC (version 0.11.2)