FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859311
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19270.10364054211479413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3201.8189894E-1229.432371145
TAGGTAG9050.027.2176465
CCGACGC16100.026.09961145
GTATTAG16300.024.4505561
GGTAGTC9250.022.7131047
CCCTAGG3106.281025E-721.0375181
TAGTCAG10250.019.7903929
GTATAGA15800.018.3449521
TTAGGTA16750.018.1658084
GTATATG23000.017.9581131
GTAGTCA12950.017.9019558
TATTAGG20150.017.2583032
ATTAGGT20050.016.9825823
TATATAC49000.016.4114573
AGGTAGT14150.015.8717746
TATACCG11050.015.7350895
GTATATA41450.015.384091
TATACAG20150.015.1006095
GTACTAG9750.014.8641141
CTGCCGC25550.014.744985145
Produced by FastQC (version 0.11.2)