FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965494
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43800.110452821262622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC16450.021.5844928
GTGTAAG15100.018.2362161
TAGGTAG13950.018.1804855
ACCGCGC7750.016.8299358
CAGTGCG7051.0913936E-1116.4453269
CTCCGCA18650.016.318579
TACAGGC15600.016.257557
GTCCTAA14950.015.995611
GCCGACC7503.092282E-1115.458607145
TAATACT47850.015.4461024
TCTCGGG13150.015.429217145
TATACAG29800.015.3191935
GTATTAG24700.015.255781
TATACTT50200.015.156445
GACCGCG7253.074092E-1014.9921837
TATATAC58600.014.9619573
ACTATGC27850.014.8306878
ATACAGG21900.014.5586196
GTATAGA24550.014.4634731
CCTAATA25350.014.2919892
Produced by FastQC (version 0.11.2)