FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402912
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26130.10874305842244743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG9900.025.6149125
GTATTAG19450.020.865021
TACACCG7950.020.0500555
GTCCTAC5759.094947E-1218.9048751
GCCCTAG4054.0840496E-717.8935011
CCTTGCG10800.017.4421739
GCCGACC3854.996662E-616.938265145
GTCCTAA10700.016.2546581
CTATACA18200.015.9238684
TATTAGG24150.015.9007912
GTATATA48750.015.7572951
TCCTTGC21200.015.37898358
CTTACAC10900.015.2883763
TCCTAAG10600.015.037542
GTGTATA18400.014.9663591
GTTAGAC14050.014.9548373
ACACCGT12150.014.907846
GTATATG27800.014.8586871
TATAGTG20000.014.8529895
TATACAG24050.014.7618585
Produced by FastQC (version 0.11.2)