FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459899
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26050.10589865681477166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10600.025.9739535
CCTAATA19900.021.4821072
GTATTAG19650.021.388421
CGGTGGG10000.019.563679145
CTAATAC23100.019.446833
TACTATG21700.018.6977187
GGTAGTC13600.018.6461627
CTAGGGT4303.7751306E-818.5346554
TAGTCAG11550.018.1918269
CTATGCA15950.018.1701979
ACTATGC20700.017.85098
ATACTAT32250.017.7483396
GCCGACC3753.888863E-617.389936145
TAATACT37400.016.8541934
ACCTAAT21450.016.5532041
TATTAGG21100.016.4830172
GTATATA46700.016.447591
TATACGG14750.016.209975
AGTGTGC8050.016.2008178
TTAGGTA19050.015.5936994
Produced by FastQC (version 0.11.2)