FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466083
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22770.15531180703957415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC4601.8007995E-1020.4760993
CTGCCGC13200.019.213224145
CCTTGCA10550.018.5427029
GCCGACC2400.001063836318.115326145
TAGGTAG5701.6370905E-1017.795695
GTCCTAG3302.5240015E-517.5669771
ACTATGC10850.017.3622238
CTAATAC12800.016.981383
TCCTTGC14050.016.5019538
ACCGTGT4007.1869654E-616.3021268
CTACACT8450.016.2914074
TATACAG16900.016.2914075
ACAGTGC6709.276846E-1116.221028
CTATGCA10850.016.0266679
GTATAAG11900.015.8324211
GTGTATA12050.015.6353371
ACTGATC8350.015.6188028
CTAGACT4301.4267938E-515.1647684
GTATATA34000.015.132141
TAATACT23050.015.0880124
Produced by FastQC (version 0.11.2)