FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063154
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47000.15343662120807508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG8400.018.1163061
TACACCG10700.016.2525565
GTATAGA26500.015.586861
GTATATA56100.015.5005831
CTAATAC25700.015.2244173
CTACACC16800.015.0951974
CTAGACT9700.014.9395784
TATACAG28700.014.8958175
GTATAGG16950.014.5357981
TATACTG38550.014.4730665
TATATAC63100.014.4684253
CCTATAC16250.013.82255653
TCTACAC19400.013.8191093
GTACAGG12650.013.7483431
GTATAAT41300.013.6859261
CTTAGAC14850.013.6619153
ACAGTGC12200.013.6603638
ATACAGC21800.013.6276696
GTATATG35100.013.625941
CTAGGGT6553.0620686E-713.2745554
Produced by FastQC (version 0.11.2)