FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568087
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40130.15626417640835374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17550.019.4041251
TATACAG22500.018.6749555
TAATACT32350.017.9158844
TATAGGG12700.017.6840022
TAGGTAG9550.017.4477165
GTATAGA16850.017.2001971
TATTAGG19200.016.602522
CCGTGTA6154.838512E-1016.4917629
TAGTCAG10000.015.9381099
GTATAGG14200.015.81782151
GTATATA42200.015.6243611
TAGTCCG16250.015.6037449
CTAATAC19050.015.5923453
GCCGACC4651.7341099E-615.581899145
ACTATGC17250.015.5391328
CCTAATA17300.015.075752
TATATAC46250.014.8810793
GTACTAG9900.014.637541
GGTAGTC10400.014.62850957
TACTATG17500.014.4891897
Produced by FastQC (version 0.11.2)