FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160427
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16950.14606692191753554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1453.5016907E-529.9864039
CCGACGC7750.028.98561145
TAGGTAG4850.023.9066855
TATACAG14200.021.9442775
CTAATAC11250.020.6119883
ATACAGG8800.019.7637676
GTATAAG8850.019.6538011
TATACGG7300.018.8613115
TACGGTG7000.018.6344097
TAGACAG6700.018.3871865
GGATCGC2050.00915111817.6749136
TATATAC27950.017.6298893
TAAGAGT8000.017.2102054
ACGGTGT7200.017.1102988
GCTGACC3002.4256705E-416.9098
TAGTCAG6003.3833203E-1016.9099
CTAGGAC3453.695255E-516.8032513
GTCTACG3052.749951E-416.6332361
GTATATA27100.016.5806271
TAGACTG6154.802132E-1016.4965865
Produced by FastQC (version 0.11.2)