FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241159
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41110.1268373442956671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC7700.020.703857145
GTATTAG24050.018.6828351
ACTATGC23300.018.0367328
TATACGG19650.017.3308985
TATACAG32000.016.529465
TATACCG16000.016.303035
TCTATAC28500.016.271253
GTATATA70450.015.9447191
GCTGCCC11850.015.899164145
CTATACT26650.015.769454
GTATAGA27500.015.2848621
TATATAC70850.015.2381483
GTACTAG15750.015.1844681
CTAATAC29800.015.0751343
CTATGCA20050.014.8168289
TACACCG9900.014.6379615
CCTATAC17850.014.613363
GTATATG38250.014.5889981
TATACTG39100.014.4545185
GTGTATG19150.014.3807331
Produced by FastQC (version 0.11.2)