FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439942
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23030.15993699746239778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12700.021.10991
GTATAGA12350.020.5347421
GTATATA29850.019.6620521
TATACCG7700.018.8177265
GCCGACC2359.240538E-418.499352145
GTATAGG9500.018.3052561
GTCCTAC3602.6327325E-618.1145761
GTATTAC13950.017.1406751
TATAGGG6807.2759576E-1217.0466462
TTATACT17000.017.0466444
TAGACTT10250.016.9634915
TATACTG16100.015.74961955
ACTATGC10600.015.7199018
TATACAG14800.015.6644855
TTAGGAC6209.542418E-915.1907613
GTATTAT23600.015.044311
TCTCGGG2900.003769800314.990853145
GTACTAC7303.4197E-1014.8886951
ACACAGC6351.3080353E-814.83192356
CTGTCCG3457.0391665E-414.6996439
Produced by FastQC (version 0.11.2)