FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864645
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27620.14812471006545483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC6400.023.7749183
CCTTGCG8250.021.9566359
GTATTAG15200.021.4533561
CAGCGCA6500.021.1797079
TCCTTGC14800.020.5620928
CTTAAGG7250.018.9887033
TATACAG18950.018.7355585
GTCCTAC4705.173206E-918.5016161
TAGGTAG7050.018.4996325
CTCCTAT10600.018.4579811
GTGTAAG9200.018.1161651
CGGTGGG7600.018.114708145
TCTACAC12150.017.8905933
CAGTCCG2851.6414227E-417.796439
TCCTAAG7800.017.6497572
GTATATG21800.016.9527441
CTAGACT5602.4756446E-916.820354
ACTATGC13400.016.7624158
CCCTAGG2600.001818443816.7226161
GCCCCGA3905.6490026E-616.7208218
Produced by FastQC (version 0.11.2)