FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808016
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27370.15138140370439201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3809.111318E-920.97566145
GTATAGA14850.020.4958271
ACTATGC13000.018.949348
CTAATAC16150.018.394243
GTATATA34050.018.303071
TAGGACG3602.6345097E-618.1138424
GCTTAGA8100.017.8931831
CTGTGCG3453.7017624E-516.800789
TACTATG16550.016.6358177
GCTGCCC7501.8189894E-1216.424578145
GACCGCG2650.002067500316.4045337
GTATTAG13500.016.1038651
TCTATAC13750.015.8084433
TAGAGCG4601.5488895E-615.7511665
CTGAGCG4151.0196087E-515.7127769
TATATAC36450.015.7036313
AGTGTGC6006.184564E-915.6982298
ATACTAT26350.015.6734566
GTATATG20400.015.630221
TACACCG5102.6409543E-715.6276275
Produced by FastQC (version 0.11.2)