FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754678
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56760.15117141869422623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG15800.019.718765
GTATTAG30200.018.2366161
GCCGACC5051.26128725E-817.217712145
GTATAGA29300.016.8181761
TATTAGG32400.016.5485782
GTATATA70700.016.194791
GTATAGG19200.015.8520581
CTGCCGC49700.014.579048145
ACCGCGC6001.0512122E-714.490618
ACAGTGC15000.014.4906098
TCTATAC29950.014.2734573
ACTGTGC14000.013.9730878
CTAATAC32700.013.95943
GTGTATA28200.013.87657451
TAGTCAG14750.013.75379859
TATACAG36500.013.6969675
GGTAGTC16650.013.4899367
GACAGTG21600.013.4174087
TATACGG19500.013.3763035
GTATTAT59600.013.3747011
Produced by FastQC (version 0.11.2)