FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015909
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76480.15247485550475495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA40050.017.9130571
TATACGG26150.017.7331035
GTATTAG38700.017.0399171
TAGGTAG19000.016.3980145
GTATAGG27150.016.0146881
TATACAG46300.015.9623065
GTATATA83150.015.9487221
TATTAGG41250.015.2817022
GTGTAAG21700.014.3597141
GTATATG51300.014.1260161
ACTCCGC15100.013.91531858
CTAATAC39150.013.8806643
CTGCCGC64350.013.850155145
CCTGGAC13200.013.722933
TCTAGAC20450.013.4639053
TCTATAC38500.013.3622143
TACGGTG24450.013.3355247
TATATAG60450.013.3046395
TAATACT72200.013.2474
ACTATGC32900.013.2137938
Produced by FastQC (version 0.11.2)