FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064673
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29810.14438121678348098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3355.5460987E-821.626917145
CCGACGC18200.020.301971145
GGTAGTC9400.019.2681967
GTATTAG18050.018.8678841
TAGTCAG9350.018.5968369
TAGGTAG10550.018.541755
CTTACAC10450.018.0258793
TCTATAC18000.017.7100433
GTATAGA17900.017.4067611
TATTAGG21450.016.8885612
TATACGG15450.016.8815945
TATACCG11350.015.9581885
GTATATA50000.015.2167461
GTATATG27150.015.2127441
GGGCCTC4401.775261E-514.81899456
GGACGGC3507.851789E-414.4896836
ACTATGC14600.014.3907898
TACACTC10600.014.3533355
GTCCTAG4552.4323273E-514.3328861
ACGGTGT12800.014.1504248
Produced by FastQC (version 0.11.2)