FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10221
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200.19567556990509735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGT100.007023753144.75986
GAGTGTG100.007023753144.75989
GGCAAGT100.007023753144.75981
CCGCTAA100.007023753144.75981
CGCTAAG100.007023753144.75982
AATAGCA100.007023753144.75987
GCTAAGG100.007023753144.75983
CCTTTGT100.007023753144.75988
AAGGAGT100.007023753144.75986
TTTATGG100.007023753144.75988
GTAATAC100.007023753144.75983
AATTGTG100.007023753144.75985
GGAGTGT100.007023753144.75988
AGGAGTG100.007023753144.75987
TTATGGA300.001893498772.37999
GGGGGGG35350.07.575831145
AAAAAGG2050.00497210267.061454140-144
TAAAAAA3150.0082037446.8933244145
TCATTAA2853.7109313E-46.603079130-134
CCGTATC3058.4534293E-46.1700897125-129
Produced by FastQC (version 0.11.2)