FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826020
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41750.14773426939653647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGC25050.019.091633145
TATACGG19200.018.492435
TATACAG34300.017.5340845
GTATATA66700.017.0573441
TCTATAC22050.016.7594873
TAGGTAG13100.016.5939245
ACCGCGC3554.7269383E-516.3287568
TACGGTG17350.016.287587
TAGACAG15800.016.0512965
ATACGGT20350.016.0231276
TATATAC76950.015.7255913
GTATAGA25550.015.5994521
TATACCG15850.015.5434995
GTATTAG21400.015.2382941
ACGGTGT16900.015.0062718
CTATACT27050.015.000994
CATATAC39700.014.419023
GTATATG38250.014.3985811
TACACCG9750.014.1204385
TATACTG37500.014.1055735
Produced by FastQC (version 0.11.2)