FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_dgy1706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_dgy1706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299684
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT19100.637337996022477No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG17790.5936252853005165No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT16900.5639273367947572No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA16100.5372325516210409No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT11240.3750617316907142No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT10660.3557080124397699No Hit
AGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTGTCCTT9040.3016510724629944No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA8150.271953123957235No Hit
CTATAGACTATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTC7260.24225517545147557No Hit
AGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTATAATGTCATTCTCC6330.21122248768703036No Hit
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC6060.2022129976909011No Hit
GTATTATGCAGCACTTCCAACCTATGGTGTACGATGAAAGTAGGTGTGTA5290.17651926696119913No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT5210.17384978844382748No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC5100.1701792554824415No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT4910.16383924400368388No Hit
CAAATATACTATCTGTTTCAGGGAAAACTCCCAGGTTCGGATGTTCAAAA4800.16016871104229788No Hit
ATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGC4670.155830808451569No Hit
CTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTGTCC4670.155830808451569No Hit
GACTAATGTTGTGGGAAATTGGAGCGATAAGCGTGCTTCTGCCGTGGCCA4590.15316132993419734No Hit
GTACTAGACTACCTTTCATCCTACATAAATAGACGCATATAAGTACGCAT4590.15316132993419734No Hit
GTCTAGTACCTCCTGTGATATTATCCCATTCCATGCGGGGTATCGTATGC4540.15149290586084008No Hit
GTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCT4510.15049185141682572No Hit
GGCCAGGACAACGTATACTCATCAGATAACAGCAATACCTGATCACTACT4400.14682131845543972No Hit
AGACTATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAA4390.14648763364076828No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG4390.14648763364076828No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC4240.14148236142069648No Hit
ATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTCAC4170.13914656771799627No Hit
GAGTAATACTAGAGATAAACATAAAAAATGTAGAGGTCGAGTTTAGATGC4100.13681077401529612No Hit
GAGTATACTAGTATAGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTA4060.1354760347566103No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC3930.13113813216588138No Hit
ATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCG3920.13080444735120994No Hit
CCTATATCTGCGTGTTGCCTGTATATATATATACATGAGAAGAACGGCAT3870.12913602327785267No Hit
ATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATGCGTACT3860.12880233846318123No Hit
GTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGA3860.12880233846318123No Hit
ATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTG3800.1268002295751525No Hit
GCTATAGATTGCATAGAAGAGTTAGCTACTCAATGCTTTTTGTCAAAGCT3790.12646654476048103No Hit
ATTATATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTG3740.12479812068712379No Hit
CTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTC3690.1231296966137665No Hit
GTATTATAGCTCATGAATGTGGCTCTCTTGATTGCTGTTCCGTTATGTGT3660.12212864216975214No Hit
GCATAATACATTGCTTAATACAAGCAAGCAGTCTCTCGCCATTCATATTT3640.12146127254040924No Hit
ACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACACGCACTATG3390.11311915217362288No Hit
GCTTAATACAAGCAAGCAGTCTCTCGCCATTCATATTTCAGTTATTTTCC3360.11211809772960853No Hit
GTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGC3360.11211809772960853No Hit
GGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGA3330.11111704328559414No Hit
ATCATATACTAAAGGAACTAATTCAATCTCAGGGAACCGTAAAGGTTTAT3320.11078335847092272No Hit
ATCCTACCCCTTGCGCTAAAGAAGTATATGTGCCTACTAACGCTTGTCTT3270.10911493439756544No Hit
GCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGC3250.10844756476822252No Hit
GTATTAAGCAATGTATTATGCAGCACTTCCAACCTATGGTGTACGATGAA3220.10744651032420817No Hit
GGGTAGGATCGATCCACTTGTATATTTGGATGAATTTTTGAGGAATTCTG3210.10711282550953671No Hit
ATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATG3200.10677914069486526No Hit
CATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACA3200.10677914069486526No Hit
TACTAGTATAGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTATAATG3200.10677914069486526No Hit
TATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGC3180.10611177106552236No Hit
ATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTG3150.10511071662150798No Hit
GCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCATGGAATGGGA3130.10444334699216508No Hit
GTAGTAAGGAGAATGACATTATAAAGCTGGCACTTAGAATTCCACGGACT3100.10344229254815071No Hit
TATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATGCGT3080.10277492291880781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG1750.062.1368527
CTCTGTG4750.059.520567
TGCAGTC2600.058.5520327
ATATCTC5100.058.278683
GAGCGCA1950.055.7638449
CTGTGCT5500.055.3582849
TCTGTGC5600.051.780718
ATCTCTG5750.051.6906555
CCCTTGA604.941856E-448.328661
TACACTC855.335549E-542.6429375
TATACCG2800.041.4245645
TAATGCA4700.040.102514
TCTCTGT7150.039.541636
CACCCGC1859.640644E-1139.19194145
ATAGCTC1701.8226274E-938.3786436
ACAGTGT951.0277529E-438.154218
TATACAG12950.038.065825
ATAGAGC2900.037.4963766
ATGCAGT4100.037.1305586
ATACAGG12500.037.1164136
Produced by FastQC (version 0.11.2)