FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549768
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC10350.021.714952145
TATACCG14650.018.8058095
TATACGG14150.018.4455725
GTATATA52550.017.7990631
GTATAGA21900.017.5473631
GTCCTAC5753.4870027E-916.3928931
GTATTAG20500.016.269871
TATACAG30950.016.1634675
CTAATAC25300.016.0477283
CACGAGC12350.015.850184145
ACTATGC20650.015.7983948
AGTGTGC8300.015.7222588
GTATACG18900.015.3454041
GTATATG31050.015.1786051
TAATACT39250.015.1464814
TCTATAC22300.014.9554353
CAGTGCG4852.6714279E-614.9478519
ACGGTGT14700.014.7953218
CTATGCA19700.014.7202199
TCCTACG6451.5932528E-814.612962
Produced by FastQC (version 0.11.2)