FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240945
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8800.023.0683145
ATGCCGG13950.021.82807145
TAGGTAG12500.021.4601125
GTATTAG25900.020.15611
CTAATAC29200.018.8696733
TATACAG37250.018.2953975
GAGCCCC14650.017.815792145
GTATAGA27150.017.3587041
GTGTAAG13950.016.6321951
GAGCGCA7601.8189894E-1216.216949
TCTATAC26050.015.8635623
ACTATGC28500.015.51738558
GTATAAG23500.015.4268031
GTATATA67500.015.0382761
CATATAC41500.015.0239433
TATACGG17900.014.9861125
TAATACT57000.014.8814274
ATACTAT53450.014.7846956
TACTATG31100.014.6863457
CCTACAC7907.094059E-1114.6833953
Produced by FastQC (version 0.11.2)