FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367976
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC15400.021.184927145
TATACAG35050.018.8232655
ATGCCGG14400.018.628351145
TAGGTAG12100.018.5745335
GTATTAG24650.017.3543741
TAATACT51950.017.3052444
TATACGG18300.017.035665
TACTATG31450.016.828197
GTATATA67450.016.769361
GTGTAAG15600.016.7321511
CAGCGTG8700.016.6665579
TATATAC73800.016.5042023
ACTATGC30350.016.4825948
TCGTGCG4058.044937E-616.1110069
CTAATAC29850.015.7873993
GTATAGA27250.015.69854451
ACAGCGT13100.015.4960828
TCTATAC26400.015.3789163
GTGTATA24100.015.3435891
ACTGTGC14100.014.911258
Produced by FastQC (version 0.11.2)