FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815277
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30800.018.8333451
TAGGTAG16000.018.1242435
TATACGG25450.017.6613455
GAGCCCC16200.017.454348145
GTATATA79550.017.0447081
TACGGTG22050.016.7680077
TATACCG20700.016.4606655
ATACGGT26900.016.1703286
ATGCCGG16800.015.967852145
ACCGCGC5503.5886842E-815.8175228
GCCCACC10500.015.191037145
ACTATGC29650.014.9150598
GTATAGG22150.014.7308211
TCTATAC30150.014.6679063
CTAATAC35350.014.56111053
TATTAGG35150.014.4380872
ACGGTGT22050.014.1377328
GACCGCG5154.9797945E-614.0770837
GTATAGA33700.013.9852951
GTATAAG32100.013.77885151
Produced by FastQC (version 0.11.2)