FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578010
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG6500.021.19336145
GAGCCCC6100.020.20592145
ACCGTGT4351.9663275E-919.9997258
TATACCG8450.019.7340815
CCGTGCC1950.0068428118.5894899
CCTACAC4354.2742613E-818.3336623
GTATATA34850.018.307941
GTATAGA13900.018.2563021
GTGTAGG5601.2551027E-1018.12591
GTATTAG13150.017.6434611
TCTATAC13150.017.0915623
CTAATAC14600.016.387283
CTATACT13850.015.1807784
CTATACA12000.015.1044374
GTATATG18650.014.7728511
TAGGTAG6952.7011993E-914.6045785
GTGTATG9450.014.5774431
TATACGG9650.014.2748685
TATATAC38050.014.10012153
CCTATAC8251.4188117E-1014.0608593
Produced by FastQC (version 0.11.2)