FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408181
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC16300.021.35129145
TATACAG33100.019.055925
GTATTAG26300.017.0935361
GTATAAG23500.016.66181
CTAATAC31050.016.3451123
TAGGTAG13900.016.1690885
AGTGTGC10400.016.0332078
ATGCCGG15450.015.955888145
GTATATA67450.015.6952041
GTATAGA27600.015.5002581
TAGACAG16700.015.1946345
GTCCCGG5354.516769E-714.9061279
TAATACT52100.014.8896574
GTATATG38200.014.6158281
ATACAGG21850.014.5991366
ACTATGC27550.014.4732818
TATAAGG25250.014.357092
CTATACA25450.014.2436364
TATACGG20600.014.0776925
CACGAGC20650.014.044636145
Produced by FastQC (version 0.11.2)