FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700421
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC26800.021.101484145
CCTAATA35700.017.668122
GTATAGA32700.017.5166471
GCCCACC16350.017.294182145
TAATACT58300.016.7880364
GTAGGAC11550.016.320223
CTAATAC41350.016.130473
ACTATGC33500.016.0148058
ATGCCGG21800.015.96386145
CTAGGAC10700.015.5839943
TATACAG38200.015.3729195
TATATAC77400.015.361643
GTATAGG24500.015.0930061
GTATAAG32950.014.74317551
CTATGCA29100.014.6992029
TACTATG34200.014.4149417
TATAAGG34750.014.3957272
GTATATA68650.014.3638221
ATACTAT57100.014.3473746
TAGACAG24900.014.2669595
Produced by FastQC (version 0.11.2)