FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838234
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15550.019.5857661
TAGGTAG6550.018.8168355
ATGCCGG7650.018.007067145
GTCCTAC3753.859308E-617.4033551
GTATAGG8500.017.062111
TATAGGG9250.016.4599462
GTGTGCA5854.3874024E-916.1107089
TACACCG4601.5389796E-615.7609045
AGGTCCG2800.002972636615.5357495
GGACTGC5806.880873E-814.9996256
CTGATCG4351.5829492E-514.9996259
ATACGGT10650.014.9761516
TATACCG9200.014.9728585
GTATAGA13100.014.9456271
TAGGTCC5354.5119486E-714.9065764
TCTATAC13650.014.8722333
ACTATGC11800.014.7453958
GGACCGT3457.0037926E-414.7097786
GTATATA29800.014.6001281
GAGCCCC9450.014.577148145
Produced by FastQC (version 0.11.2)