FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674421
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG20450.021.2713625
ATGCCGG8500.019.619375145
GTCCTAG3001.1056964E-519.334461
ATACAGG14650.019.3003146
CTATACA13600.018.658064
ACCGTGC2359.205542E-418.5100578
GTATATA39050.018.0100141
GTACTAG8950.017.8222681
ACCGTGT4205.9511876E-717.2613648
TCTATAC14850.017.0875173
AGACGGC14600.016.388432145
CACGAGC8100.016.112532145
ACACAGC7201.6370905E-1116.1110886
CTAGGGT4501.2236615E-616.1110884
CACTGTG6306.7484507E-1016.1106077
TACACCG5503.5826815E-815.8181585
GAGCCCC6951.6189006E-1015.648861145
TAGGACT6501.0495569E-915.6153624
CAGTGCG3254.450724E-415.6148969
TATACGG10700.015.584095
Produced by FastQC (version 0.11.2)