FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394226
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG9000.021.7497125
GTATAGG12700.021.1235311
TAGGTAG9750.020.0766565
TAGGACC9050.017.6240774
GCCCACC7000.017.607645145
CCTAATA20250.017.1856752
AGACGGC23000.017.022224145
TAGTCAG9450.016.8777319
GGTAGTC10450.016.6501487
GTATATA46900.016.077891
GTATTAG19400.016.0707171
ACTGTGC9950.016.0296048
GAGCCCC9500.016.026772145
TATATAC48600.015.8128783
TATACAG23100.015.6924335
CTAATAC21300.015.6573973
GTATAGA20550.015.5242051
CTATGCA15600.015.3360169
ATACTAT34700.015.0427146
GTATAAG19150.014.7660511
Produced by FastQC (version 0.11.2)