FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595579
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG16000.020.8440575
CACGAGC13700.016.935205145
TATACAG25250.016.653715
TAGGACT9350.016.283664
GTATAGG12950.016.237011
TAGGTAG11300.016.0400585
GTATATG29250.015.61681651
ACGGTGT14600.015.3931718
CTAGGGG3808.413542E-515.2633813
ATACGGT17600.015.2414066
ATACAGG15700.015.2387836
CATATAC28750.015.1306563
GTCCCGG4351.583721E-514.9993529
GAGCCCC10650.014.977322145
TGGACCG6001.0422991E-714.5002135
ACTGGTC13100.014.3886928
TCTATAC21700.014.36656953
AACCGCG4051.4481979E-414.3203717
GTATAAT32050.014.0262471
TTATACT32700.013.9680934
Produced by FastQC (version 0.11.2)