FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053809
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA15800.018.8162121
TATACAG20850.018.0821425
GTATATG25850.017.6719911
GCCCACC7050.017.483282145
AGTCCCG3052.743868E-416.6390938
TAGGTAG7451.8189894E-1216.544185
CACGAGC11900.016.450533145
GTCCCGG3103.106055E-416.370729
CTATACT18700.016.2839684
GTGTAAG9600.015.861841
CCTATAC11100.015.6762033
GTATTAG16250.015.617811
TCGGTCG2800.002973138615.53548059
TACGGTG11800.015.3599387
CATATAC29100.014.94895653
GTGTACC9750.014.871579
GTATATA45450.014.8372891
TATACGG13200.014.8300445
TCTATAG13200.014.8300443
GAGCCCC8351.0913936E-1114.761333145
Produced by FastQC (version 0.11.2)