FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881524
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC9300.023.388027145
CACGAGC17650.018.485153145
GTATAGA22550.018.3276231
TATACAG29050.018.218685
GTCCTAG6850.017.9943521
ATGCCGG17550.017.764238145
GTATTAG21900.016.5540311
TATACTG32850.016.5525935
CTATACA22450.016.4700164
GTATAGG17650.016.4321021
ACTATGC20550.016.228238
AGACGGC27450.015.847625145
CCTTGCG13350.015.2055419
TAATACT44400.015.0225984
GTGTAGG9200.014.9742011
GTGTAAG14100.014.9127161
CTAATAC26300.014.8860063
TATACGG17550.014.8718685
TATATAC61600.014.8296193
GTATATG32300.014.8156011
Produced by FastQC (version 0.11.2)