FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506244
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG5300.024.6255511
GAGCCCC12000.021.751268145
TATACAG30050.019.5416185
GTATTAG17050.019.137161
TCTATAC22350.017.5160543
TACACAG13450.017.2483565
TATACGG14800.017.144565
GTATATA57750.016.9500541
TACAGGC14750.016.7111727
CTAGGGT5652.7685019E-916.6807254
AGACGGC24100.016.24576145
TACACCG8500.016.2052125
ATACAGG21200.016.0724476
TACGGTG13550.016.0509957
TATACTG31300.015.9817675
TAGTACT18650.015.1602314
GCCCACC8751.8189894E-1214.915156145
GTATAGA21950.014.8650831
GTATAAG19150.014.7667591
CTATACA20650.014.74515154
Produced by FastQC (version 0.11.2)