FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310158
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1950.00684475718.5890129
GTATATA50550.018.503161
AGACGGC20600.017.950655145
ATGCCGG12350.017.025835145
TAGACAG13650.016.995675
GTATAAT34050.016.8223761
TCGTGCG3956.344264E-616.518349
TATACAG26450.016.4454785
CTATGCA14150.016.3951159
TACACCG8000.016.311865
GTATAGA19100.015.9438531
CAGCCGG4601.5400637E-615.7602519
CACCGTG7452.7284841E-1115.56985957
TAGGTAG9800.015.5351055
ACTATGC15100.015.3636378
GTATATG30700.015.3515531
GAGCCCC8551.8189894E-1215.264541145
GTACTAG10500.015.1918531
ACCGTGC5354.515532E-714.9059568
CTAGGAC5959.413088E-814.6212743