FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815264
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10600.023.940685145
ACTATGC16750.020.342468
TACACCG9150.019.0166365
CTAATAC23300.018.980593
TAGGTAG10000.018.125235
CTATGCA15750.017.4913549
GTATAGA22100.017.3886431
GAGCCCC11650.016.803984145
GTATATA50000.015.5165751
GTCTAGG9400.015.4270981
GTATAGG16050.015.3598161
TATACAG26450.015.3499125
TAATACT41200.015.309394
GTATTAG21550.015.1407491
ATGCCGG16400.015.031747145
GGTAGTC10250.014.8530567
TATTAGG27500.014.7640882
AGACGGC27150.014.688155145
CACAGTG11900.014.6212567
TAAGACA23750.014.3472964
Produced by FastQC (version 0.11.2)