FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921791
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA46800.019.0555631
CTGATCG5101.4135367E-817.0584569
GTATAGA17050.017.0097581
CCTATAC10800.016.7824823
GTATAGG11100.016.3297521
TATACAG22200.016.3289035
GAGCCCC8000.016.312998145
TATACGG11600.016.2500725
TATATAC46200.015.6927123
CTAGACA9850.015.4569224
ACGGTGT10600.015.0468458
CTACACC10650.014.9765944
CCCCTGG4401.7622913E-514.8303831
CCCGGTC2950.004207767514.7454458
TACTCCG5506.169266E-714.5000655
GGACAGC5506.171085E-714.4996886
GTCCTAG5104.4939097E-614.216491
GAGTCCG5658.3624946E-714.1147399
CATATAC24700.014.0891313
TACGGTG10900.013.9675897
Produced by FastQC (version 0.11.2)