FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159213
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA23250.019.9583571
TAGGTAG11000.019.7722035
TATACGG13550.019.2614845
GCCCACC15450.017.832653145
ACTCCGC9700.017.190548
TAATACT38000.016.9798154
ATCTCCC18500.016.852242145
TATACAG25600.016.7085445
GTATTAG21250.016.0363941
GTATATA47400.015.75531
CCTAATA25700.015.51542
TCTATAC23200.015.3120963
TCCGCGT3901.05023195E-414.8716378
GTGTCCG5455.5682904E-714.6328739
TACTATG23450.014.5307637
TATACCG13550.014.4461135
GTATAGG16350.014.1905761
ACTATGC22100.014.1061868
CTAGGAC7254.9094524E-913.999633
TATACTG32900.013.8826115
Produced by FastQC (version 0.11.2)