FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108391
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC9350.020.161154145
GTATATA49400.018.0526961
GTATTAG16450.017.6302321
CCTAGAC6603.6379788E-1217.575143
TATACAG26850.017.0105745
GTATAGA18450.016.8980271
CCCTATA10850.016.7048782
GTATAGG15700.016.6251961
TAATACT35900.015.9534794
ATACAGG16100.015.7603166
TCTATAC18000.015.7077823
TAGGGCG2850.00334564915.2626215
TATACGG14250.015.2626215
TCCTAGA11000.015.1589162
GGAGCGG3355.603085E-415.1490798
TACACAG12550.015.0193935
TGCCGTG18350.015.014164145
ATACAGT20850.014.9515146
TACGGTG11400.014.6270267
ACGGTGT11400.014.6270268