FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002528
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT16550.022.778974
GTGCGCG2003.102447E-421.750629
TAGACTG5400.021.4810245
CTATGCA5900.020.8904279
CTAGGGT2857.0992955E-620.3504434
TATACAG12000.019.9370775
TTAGGAC3351.3146855E-619.4771983
CTTAAGG4102.167144E-819.4508063
ATACAGG8350.019.1013876
TATACCG5056.184564E-1018.6629685
ACTGTGC4705.133188E-918.5111668
GTATAAT13750.018.4559941
TAGGTAG3953.1233867E-718.3540385
CCTCGGA2400.001059509718.1255199
GAGCCGA2400.001059509718.1255199
CCCACAC4003.5643643E-718.1246133
GTATATA27100.017.925761
GAGCCCC5301.1787051E-917.783525145
TATATAG17000.017.484925
GTATACA13000.017.2898161
Produced by FastQC (version 0.11.2)