FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303865
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA9650.021.0384792
CCCTTAG6900.019.9666291
TAGAGTG6101.8189894E-1219.0169325
GCTATAC5801.0913936E-1118.751973
GTATAGA10200.018.4830421
GAGCGCA3151.6854248E-518.4111029
CCCTATG3552.2881832E-618.3822122
GTATATG16600.018.3460041
CACGAGC10800.017.454866145
CTTACAC6653.6379788E-1217.445443
GTATATA30000.017.4024931
TATACTG14550.016.942065
TCTAGAC6104.2564352E-1016.641093
ACTATGC9150.016.6379018
CTACACC7850.016.625954
AGTGTGC4851.4815123E-716.4418748
TACTATG11650.016.1788547
GAGTGTG5904.9094524E-915.973197
CTAGACT5453.1959644E-815.9649794
ACAGGCC4109.036969E-615.9132548
Produced by FastQC (version 0.11.2)