FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464525
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGG3809.05311E-920.986628
GAGCCCC5650.020.53216145
ACTATGC9800.019.9742778
GTATAGA13550.019.2631051
GTATTAG11750.019.1287631
GTATATG17550.019.0039461
GCCCACC4455.54046E-817.92238145
CTAATAC13000.017.8465733
GTGTCCG2550.001591583817.0586419
GTAGACA7700.016.9478724
ACAGCGT6857.2759576E-1216.9341268
TATACGG10300.016.8930245
GTATAGG7850.016.6251621
GTGTATA11800.016.5899411
CTACACA7001.0913936E-1116.5712554
TAATACT21550.016.4847454
CTATGCA9400.016.1966389
GTCTAGA4951.866174E-716.1120411
TCTACAC9450.016.111493
TACACTG10350.016.110945
Produced by FastQC (version 0.11.2)