FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036298
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG7600.022.8975335
TACACCG3304.6842615E-821.972385
ACTGTGC4851.6370905E-1120.9273628
GTATTAG8300.020.0947551
GACAGCC4402.2646418E-919.772287
GAGCCCC4152.4894689E-819.21736145
TAGACAG6450.019.110865
CCCTATA6101.8189894E-1219.0196342
ACCTCGC2307.963422E-418.9126158
TACGGTG6201.8189894E-1218.7092557
GGACCGT2359.1987953E-418.5111146
CTTAGAC4506.28188E-817.7252433
TATACAG10650.017.7016935
CTAATAC9100.017.5304583
GGTAGTC4557.1431714E-817.5270757
CTATGCA7900.017.4363149
GTATATG14250.017.3020291
TCCTTGC11000.017.1359758
TTAGACG3403.2412296E-517.061734
CCTATAC6404.5474735E-1116.9950833
Produced by FastQC (version 0.11.2)