FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848755
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8400.022.4418415
GTATTAG13150.019.8491151
ACAGTGC8000.018.1246328
TAGTCAG7750.017.7738329
GTATAGA12900.017.423541
CCCTTAC7500.017.4010581
GACCGAC2600.001812763316.7304297
TCCTATA12950.016.236072
TATATAC39850.016.0105783
AGGTAGT11900.015.84136
GGTAGTC8055.456968E-1215.3102477
ACCGTGC3355.603E-415.1489468
TATAGTG15600.014.87275
GCCCACC6351.29602995E-814.84302145
CTATGCA11300.014.7563379
TAGGCGT4953.297344E-614.6473544
CAGTGCA10450.014.5690819
TATTAGG15650.014.3615062
ACTATGC13150.014.334318
TAGGACA8652.0008883E-1114.2494214
Produced by FastQC (version 0.11.2)