FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387994
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC24050.021.404285145
GTATTAG30000.018.1256051
GCCCACC15550.017.717834145
GTATATA68550.016.922521
GTATAGA31800.016.4156441
TAGGTAG14800.016.1653425
TATTAGG35700.016.0434042
TATACAG41350.015.9550655
TATACCG21900.015.8902185
CTAATAC36200.014.8202623
TCTATAC33900.014.7564573
GTATAGG28400.014.5515421
CTATGCA26600.014.44514759
ATGCCGG40950.014.341302145
CAGCGTG13350.014.1195179
TACACTG26250.014.0855435
TACACAG25850.014.023045
ACACCGT19650.014.0201866
TAGGACA17600.014.005514
TACACCG12950.013.9959695
Produced by FastQC (version 0.11.2)