FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132139
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA23000.021.4346549
CCTAATA28850.020.1043532
CTAATAC33350.019.130323
ACTATGC33650.018.7443168
TAGGTAG13850.017.7981595
TATACCG14700.017.7554265
TATACAG32750.017.7102475
TACTATG31200.017.427787
TAGTCCG31100.017.0175829
ATACTAT45900.016.7428196
TATATAC75500.016.4204313
GCCCACC15750.016.112185145
TAATACT56550.015.8975324
GTATAGA28100.015.7396731
TCTATAC25650.015.5457153
GAGCCCC28450.015.291001145
ACCTAAT32600.015.123891
GTATAAA50050.015.0661211
CAGATCC22700.015.0109239
CACGAGC29500.014.746744145
Produced by FastQC (version 0.11.2)