FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n01_bc_c22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_bc_c22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4152471
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA27000.017.1858581
TATACAG34700.017.1326165
ACCGCGC11800.015.9742398
TATATAC72450.015.9110213
TAGGTAG14600.015.8904595
TATACCG16650.015.6757235
GACCGCG10200.015.6369257
ACTCCGC19700.015.4565288
ATGCCGG44200.015.091087145
GTGTAAG17900.014.986621
TAATACT51400.014.8103554
GAGCCCC30000.014.742243145
GTATTAG25850.014.5847091
TATACTA37750.014.40401655
ACTATGC33250.014.3906748
GTATAAA53500.014.3650471
TATAGTG29350.014.327135
TATTATA104850.014.1750542
CTCCGCA21000.014.1544649
CCGCGCA13350.014.1195539
Produced by FastQC (version 0.11.2)